Allergy CRD LBL & Allergy by Miccroarray

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Ara h 1

Ara h 2

Peanut allergen components

Allergen Source

Ara h 9

Ara h 6

CCD

Ara h 3

Ara h 8

Without Component Testing

Allergy Diagnosis is Incomplete

Identify true allergen sensitization with certainty

Increasing allergenic potential & risk of severe reactions

CCDs

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PR-10

nsLTPs

Storeage

proteins

α-lactalbumin Bos d

4-Heat labile

HIGHER RISK of

reaction to fresh milk

LOWER RISK of

reaction to baked

milk

Patient likely to

“outgrow” milk allergy

β-lactoglobulin

Bosd 5 Heal labile

HIGHER RISK of

reaction to fresh milk

LOWER RISK of

reaction to baked

milk

Patient likely to

“outgrow” milk allergy

Casein Bos d 8

Heat stable

HIGHER RISK of

reaction to all forms

of milk

Patient unlikely to

“outgrow” milk

allergy

• Prognosis and understanding of cross-reactivity

• Differentiate between primary allergen sensitisation and

reaction to cross-reacting components

These protein share a high degree of

amino acid sequence similarity (28%-67%)to Bet v1

Protein

Birch Bet v 1 homologues (pathogen-related proteins 10)

Nomenclature

Protein fraction

Apple

Mal d 1

Bet v 1 homologue

Cherry

Pru av 1

Bet v 1 homologue

Apricot

Pru ar 1

Bet v 1 homologue

Pear

Pyr c 1

Bet v 1 homologue

Celery

Api g 1

Bet v 1 homologue

Parsley

pcPR 1 and 2

Bet v 1 homologue

Carrot

Dau c 1

Bet v 1 homologue

Milk Allergy2

Bet v 1

Bet v 2

Bet v 3

Bet v 4

Bet v 6

Bet v 7

CRD allows for a more

accurate allergen

immunotherapy (AIT)

screening

Explain Oral Allergy Syndrome

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seems to be most

effective in patients

sensitised to Bet v 1

(major birch component)

Tree pollen

Fruits

Vegetables

Hazel

Nuts

Spices

Bet v 1

300 individual Allergens

& Components

Microarray

Nanotechnology

��

molecular Allergens

No cross reactivity

AI driven automated report

Allergy component testing (CRD) by Microarray

4th generation allergy testing technology

Reference-

1- AmericanAcademyof Allergy, Asthma & Immunology [Internet].Milwaukee, WI: AmericanAcademyof Allergy, Asthma & Immunology; 2021. Available from:https://www.aaaai.

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2-Hochwallner et al.Cow's milk allergy: from allergens to new forms of diagnosis, therapy and prevention. Methods. 2014 Mar 1;66(1):22-33. doi: 10.1016/j.ymeth.2013.08.005.

Epub 2013 Aug 15. PMID: 23954566; PMCID: PMC3969108.

3-Biedermann, et al. Birch pollen allergy in Europe. Allergy. 2019; 74: 1237– 1248. https://doi.org/10.1111/all.13758

178

Molecular

Allergen

117

extract based

allergen

295

total

allergen

Egg white

Gal d_white

� 0.10

Egg yolk

Gal d_yolk

� 0.10

Egg yolk

Gal d 1

Ovomucoid

� 0.10

Gal d 2

Ovalbumin

� 0.10

Gal d 3

Ovotransferrin

� 0.10

Gal d 4

Lysozym C

� 0.10

Gal d 5

Serum Albumin

� 0.10

Egg

Test code

Test name

Price

Schedule

Method

A0680

ALLERGY COMPONENT

TESTING (CRD)

4,900

GH�

Daily:- 9.00 am, Next day 5 pm

Multiplex Nanotechnology

Assay (4th Generation)

BLOOD TESTS • DIAGNOSTICS • WELLNESS

For more details visit us at www.metropolisafrica.com Join us on MetropolisLab and MetropolisLab

For more information please contact us at: +233 244 405922/543 026795

178

Molecular

Allergen

117

extract based

allergen

295

total

allergen

Egg white

Gal d_white

� 0.10

Egg yolk

Gal d_yolk

� 0.10

Egg yolk

Gal d 1

Ovomucoid

� 0.10

Gal d 2

Ovalbumin

� 0.10

Gal d 3

Ovotransferrin

� 0.10

Gal d 4

Lysozym C

� 0.10

Gal d 5

Serum Albumin

� 0.10

Egg

Test code

Test name

Price

Schedule

Method

A0680

ALLERGY COMPONENT

TESTING (CRD)

4,900

GH�

Daily:- 9.00 am, Next day 5 pm

Multiplex Nanotechnology

Assay (4th Generation)

BLOOD TESTS • DIAGNOSTICS • WELLNESS

For more details visit us at www.metropolisafrica.com Join us on MetropolisLab and MetropolisLab

For more information please contact us at: +233 244 405922/543 026795

178

Molecular

Allergen

117

extract based

allergen

295

total

allergen

Egg white

Gal d_white

� 0.10

Egg yolk

Gal d_yolk

� 0.10

Egg yolk

Gal d 1

Ovomucoid

� 0.10

Gal d 2

Ovalbumin

� 0.10

Gal d 3

Ovotransferrin

� 0.10

Gal d 4

Lysozym C

� 0.10

Gal d 5

Serum Albumin

� 0.10

Egg

Test code

Test name

Price

Schedule

Method

A0680

ALLERGY COMPONENT

TESTING (CRD)

4,900

GH�

Daily:- 9.00 am, Next day 5 pm

Multiplex Nanotechnology

Assay (4th Generation)

BLOOD TESTS • DIAGNOSTICS • WELLNESS

For more details visit us at www.metropolisafrica.com Join us on MetropolisLab and MetropolisLab

For more information please contact us at: +233 244 405922/543 026795

178

Molecular

Allergen

117

extract based

allergen

295

total

allergen

Egg white

Gal d_white

� 0.10

Egg yolk

Gal d_yolk

� 0.10

Egg yolk

Gal d 1

Ovomucoid

� 0.10

Gal d 2

Ovalbumin

� 0.10

Gal d 3

Ovotransferrin

� 0.10

Gal d 4

Lysozym C

� 0.10

Gal d 5

Serum Albumin

� 0.10

Egg

Test code

Test name

Price

Schedule

Method

A0680

ALLERGY COMPONENT

TESTING (CRD)

4,900

GH�

Daily:- 9.00 am, Next day 5 pm

Multiplex Nanotechnology

Assay (4th Generation)

BLOOD TESTS • DIAGNOSTICS • WELLNESS

For more details visit us at www.metropolisafrica.com Join us on MetropolisLab and MetropolisLab

For more information please contact us at: +233 244 405922/543 026795

COMPONENT RESOLVED DIAGNOSIS

A milestone in allergy diagnosis by Metropolis

Egg allergen components

Gal d 3

Gal d 4

Gal d 1

Gal d 2

Allergen Source

Without component testing,

allergy diagnosis is incomplete7

178

Molecular

Allergen

117

extract based

allergen

295

total

allergen

Egg white

Gal d_white

� 0.10

Egg yolk

Gal d_yolk

� 0.10

Egg yolk

Gal d 1

Ovomucoid

� 0.10

Gal d 2

Ovalbumin

� 0.10

Gal d 3

Ovotransferrin

� 0.10

Gal d 4

Lysozym C

� 0.10

Gal d 5

Serum Albumin

� 0.10

Egg

Test code

Test name

Price

Schedule

Method

A0680

ALLERGY COMPONENT

TESTING (CRD)

4,900

GH�

Daily:- 9.00 am, Next day 5 pm

Multiplex Nanotechnology

Assay (4th Generation)

BLOOD TESTS • DIAGNOSTICS • WELLNESS

For more details visit us at www.metropolisafrica.com Join us on MetropolisLab and MetropolisLab

For more information please contact us at: +233 244 405922/543 026795

Food Allergy: A Growing Public Health Burden1

17%

Rate of self-reported food adverse reactions2

Prevalence of a food aller��by an oral food challenge2

Food allergy is referred to as the “second wave”

of the allergy epidemic, following asthma1

Standard allergy

diagnostics

Component-resolved diagnostic

(CRD) testing

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against casein plays a

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Retains strong

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boiling at > 90°C

Casein4

Very resistant

to high

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WHY IS CRD IMPORTANT IN FOOD ALLERGY?

Milk Allergy

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Molecular allergens identified in cow’s milk2

Bos d 8 (Casein)

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Casein Family

(coagulum: has approximately 80% of

the cow’s milk proteins)

Whey

(sensible to heating, lose IgE binding

after 15–20 min of boiling at >90ºC)

Bos d 4 (Alpha-lactalbumin)

Bos d 5 (Beta-lactoglobulin)

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Bos d lactoferrin

��2

Hen Eggs Allergy

Major allergen components of hen eggs

nGal d 1

(Ovomucoid)

Highly allergenic,

correlated to high

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��2

nGal d 2

(Ovalbumin)

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for clinical reaction

to raw or slightly

heated egg and

certain vaccines2

Peanut Allergy

Peanut allergen components2

Stable Proteins

�

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�

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�

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�

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�

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Labile Proteins

�

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to whole peanuts�

Serum-specific IgE

to Ara h 2 in

peanuts

Isolated sensitization

to Ara h 8

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birch pollen allergen

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milder or local

��2

Cross‐reactivity between Birch Pollen and Food

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hypersensitivity reactions to food sources6

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��6

Bet v 1

Bet v 2

Bet v 3

Bet v 4

Bet v 5

Bet v 6

Bet v 7

Food allergens cross-reactive with Bet v 15

Almond

Apple

Apricot

Carrot

Celery root/

tuber

Kiwi

Peanut

Peach

Pear

Plum

Soyabean

Cherry

Hazelnut

Pru du 1

Mal d 1

Pru ar 1

Dau c 1

Api g 1

Pru av 1

Cor a 1

Act d 8

Ara h 8

Pru p 1

Pyr c 1

Pru d 1

Gly m 4

Food

Bet v 1

Homolog

Food

Bet v 1

Homolog

WITHOUT COMPONENT TESTING,

ALLERGY DIAGNOSIS IS INCOMPLETE7

Benefits of CRD9

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diagnostic accuracy

��

cross-reactivity

��2

4th generation allergy testing technology7

��

No cross-reactivity�

��

report�

Allergy Component Testing (CRD) by Microarray

When to use CRD?8

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unanticipated allergen

triggers

Food allergy

Polysensitization

Pollen and plant food

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Allergen immunotherapy

Anaphylaxis

Cofactor-enhanced food-

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Cyn d

Cyn d 1

Guide dot 1

Guide dot 2

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Guide dot 4

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178 Molecular

Allergen

117 extract

based allergen

295 total allergen7

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Metropolis offers CRD Testing by Microarray

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20000

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��th��

Test code

Test name

Price

Schedule

Method

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CRD in Immunotherapy

CRD: A Milestone in Food Allergy

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allergic reactions2

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versus secondary

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Predicts the

evolution of the

allergic process2

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of each patient and in

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RISK

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1 2 3 4 5 6 7 8 9 10 11 12